Preparative separation of DNA-ethidium bromide complexes by zonal density gradient centrifugation
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چکیده
منابع مشابه
Preparative separation of DNA--ethidium bromide complexes by zonal density gradient centrifugation.
Restriction enzyme fragmentation and recombinant DNA technology offer powerful tools with which to study gene structure, organization, and expression. Frequently in these studies the need arises to purify on a preparative scale a specific DNA molecule from a mixture. The two most commonly used procedures in such purification are isopycnic centrifugation in dye-salt gradients and gel electrophor...
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Several hybrid lines between human and mouse somatic cells, containing one or two complements of mouse chromosomes and a reduced complement of human chromosomes, have been examined for the presence of mouse and human mitochondrial DNAs. For this analysis, advantage was taken of the fact that these two types of mitochondrial DNA have a buoyant density difference in CsCl gradients of 0.008 g/cm(3...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 1980
ISSN: 0003-2697
DOI: 10.1016/0003-2697(80)90177-3